in vitro anti-hiv-1 activities of kaempferol and kaempferol-7-o-glucoside isolated from securigera securidaca

previously, we reported that the kaempferol and kaempferol-7- o -glucoside isolated from securigera securidaca showed potent anti-hsv activity. in the present study the anti-hiv-1 activities of kaempferol and kaempferol-7- o -glucoside are investigated at different concentrations (100, 50, 25 and 10 µg/ml) using hiv-1 p24 antigen kit. real-time polymerase chain reaction (rt-pcr) assay was also used for quantification of full range of virus load observed in treated and untreated cells. according to the results of rt- pcr, tested compounds at a concentration of 100 µg/ml exerted potent inhibitory effect. time of drug addition experiments demonstrated that these compounds exerted their inhibitory effects on the early stage of hiv infection. the results also showed potent anti-hiv-1 reverse transcriptase activity. antiviral activity of kaempferol-7- o -glucoside was more pronounced than that of kaempferol. these findings demonstrate that kaempferol-7- o -glucoside could be considered as a new potential drug candidate for the treatment of hiv infection which requires further assessments.